The order mandates the Secretaries of the Interior, Agriculture, and Health and Human Services, along with the Administrators of the Environmental Protection Agency and General Services, to develop and implement a campaign within 60 days. This initiative aims to reduce invasive mosquito populations by 90% and tick populations by 50% in D.C. by 2028, utilizing smart land management, non-chemical controls, and advanced biotechnologies like sterile insect techniques and safe genetic modification.